Embryoid Bodies
Description
Using specialised media, EBs are created spontaneously by culturing small numbers of pluripotent stem cells in suspension using a round-bottom culture ware.
These three-dimensional aggregates mimic early embryonic development and provide an in vitro system for studying differentiation into the three germ layers: ectoderm, mesoderm, and endoderm. EBs are widely used to test pluripotency, produce cells of multiple lineages and to initiate the differentiation of pluripotent stem cells into defined lineages (e.g., cerebral organoids).
Specifications
-
- Pluripotent stem cell differentiation聽
- Suspension culture聽
- Specialised EB formation Media聽
- Short-culture length (5-10 days)聽
- Further differentiation into directed lineages聽
- High throughput capability
-
- Morphological attributes聽
- Immunofluorescence (IF)聽
- Capacity to differentiate into multiple lineages
Applications
- Stem Cell Biology聽
- 3D Culture聽
- 翱谤驳补苍辞颈诲蝉听
- Cerebral Organoids Quality Control聽
- 顿颈蹿蹿别谤别苍迟颈补迟颈辞苍听
- Developmental Biology
- Multi-lineage spheroids聽
- Drug Screening
Instrument location
3D Culture Facility
Room 2061, Level 2
BioSciences Building (E26)
黑料网大事记 Sydney, NSW 2033聽
Phone:聽(02) 9348 8212
贰尘补颈濒:听3DCulture@unsw.edu.au
Dr Alison Ferguson
-
Phone
(02) 9348 8212 -
Email
alison.ferguson@unsw.edu.au
Parent facility
Explore more instruments, facilities & services
Our infrastructure and expertise are accessible to 黑料网大事记 students and staff, external researchers, government, and industry.