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pDNA and linDNA Production, Purification and Quality Control

Delivered in partnership with the ºÚÁÏÍø´óÊÂ¼Ç Recombinant Products Facility

Scientists at work at the RNA Accelerator – the core facility within the ºÚÁÏÍø´óÊÂ¼Ç RNA Institute designed to expedite the discovery, manufacture and translation of RNA-based therapeutics and other products.

This course provides comprehensive theory and hands-on training from e. coli based production of plasmid DNA (pDNA) through to purified, linearised DNA template (linDNA) as a starting material for mRNA production.

The course covers multiple techniques relevant from the research bench up to large-scale bioprocessing as well as basic quality control to quantify and analyse product quality. The course includes an overview of Quality Management Systems (QMS) requirements, Bioprocess Engineering and Process Development, and insights into process tech transfer to large-scale GMP manufacturing facilities.

Course Details

¶Ù²¹³Ù±ð:ÌýMarch 2-6, 2026
Length: 5 days
Location: ºÚÁÏÍø´óÊÂ¼Ç Kensington campus
Fees: $5,000 per participant

Course Objectives

Give participants a combination of theory and practical, hands-on experience in producing, purifying and analysing the quality of plasmid DNA and linearised DNA template for mRNA therapeutics.

Learning Outcomes

Upon successful completion of the course, participants will:

  • Have a fundamental understanding of mRNA-LNP therapeutics manufacturing processes.
  • Have a sound understanding of plasmid DNA and linearised DNA template manufacturing processes.
  • Have a sound understanding of plasmid DNA and linearised DNA template purification processes.
  • Have a sound understanding of plasmid DNA and linearised DNA template quality control methods.
  • Gain hands-on experience in aseptic, pDNA and linDNA handling techniques.
  • Gain hands-on experience in production of pDNA and linDNA.
  • Gain hands-on experience in purification of pDNA and linDNA.
  • Gain hands-on experience in quality control of pDNA and linDNA.
  • Have a fundamental understanding of Quality Management Systems (ISO 9001, GMP).
  • Have a fundamental understanding of Bioprocess Engineering and Process Development concepts.
  • Have a fundamental understanding of tech transfer from bench research to large-scale GMP manufacturing facilities.

Participants will have the option to receive a Certificate of Attendance (not assessed) or a Certificate of Completion (assessed).
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